488nm Laser (Blue) DetectorFilterMirrorDye A 710/50 685 LP PerCP, PerCP-Cy5.5 B 530/30 505 LP FITC, GFP, CFSE, Alexa Fluor 488 C 488/10 - SSC 561nm Laser (Yellow/Green) DetectorFilterMirrorDye A 780/60 750 LP PE-Cy7 B 710/50 685 LP PE-Cy5.5 C 670/30 635 LP PE-Cy5 D 610/20 600 LP PE-Texas Red, PE-CF594 E 585/15 - PE 640nm Laser (Red) DetectorFilterMirrorDye A 780/60 750 LP APC-Cy7, APC-efluor

Designed for limited space and boundless potential. The BD LSRFortessa™ X-20 cell analyzer delivers high-performance multicolor analysis with the most compact footprint in its class at just 30" x 29" (76.2 x 73.7 cm) and a height of 30" (76.2 cm).

With up to four different laser configurations and 14 parameters, the cytometer is fully optimized to work with BD Horizon Brilliant ™ dyes, so you can focus on what matters most—your research. Optical Filters | BD - Filter hold. LSR II; Aria II; Canto II; Fort. | Order now at AHF - Wide selection Custom-specific Expert advise We set up our configuration such that the parameter names specify the laser, detector and filter, 642 C 675/20, etc.

Bd fortessa filters

Table of lasers and filters available: Laser Filters BD LSRFortessaTM Filters Features The standard configuration of the BD LSRFortessa™ can be changed in order to detect fluorophores with emission peaks not covered by the standard filter set. In the following list you can see all additionally available filters with some fluorophores, covered better by the alternative filter… LSR Fortessa . The LSR Fortessa offers 5 lasers and is able to detect up to 18 parameters (fluorochromes) at the same time! Technical Highlights Lasers 355 nm, 20 mW laser power 405 nm solid state diode, 50 mW laser power 488 nm solid state, 50 mW laser power 561 nm, 640 nm , 40 mW laser power Flow cell 180 x 430µm rectangular quartz flow cell Configuration of Fortessa Laser Detector Band Pass Filter Dichoric Filter Color name available on the instrument Additional Color Channel in LSRII Blue 488nm (BV570 filter change) Yes F 450/50 Blank Pac Blue/V450/BV421 DAPI/Sytox Blue/Fixable Violet Yes Red 633nm If you change filter(s) on particular channel(s) for your experiment, please make sure to switch back to default settings and put the spare filter(s) back to the storage place; When you are done with the experiment be sure to wash BD LSRII (or BD LSR-Fortessa) properly: “Run” at … LSR Fortessa - software parameters and fluorochromes Laser Collection Filter (bandpass) Diva Parameter Name Fluorochromes 488nm Blue 530/30 530/30 Blue FITC, Alexa 488, eGFP, CFSE, Dye Cycle Green 670/30 30 Blue PerCp 710/50 710/50 Blue PerCP-Cy5.5, PerCp-eFluor 710, PerCp provide easier access to bandpass filters and mirrors, simplifying changes to experimental setup. To improve experimental efficiency, the optional BD™ High Throughput Sampler (HTS) allows sample loading from 96- or 384-well microtiter plates for screening assays. Boundless Potential The BD LSRFortessa Analyzer 4 5 BD High Throughput Sampler (HTS) BD FACS LSR Fortessa User Manual Flow Cytometry Unit – Biomedical Core facility Technion Faculty of Medicine For help please call: Amir Grau 054-210-0778 Yaakov Sakoury 050-749-8565 Ofer Shenker 052-456-8846 A. Starting Up 1. Turn on the instrument: a.

Fortessa flödesanalysator (BD Biosciences) användes för TagBFP mättes med användning av en 405 nm laser och ett 450/50 filter med ett fotomultiplikatorrör

The LSR Fortessa offers 5 lasers and is able to detect up to 18 parameters (fluorochromes) at the same time! Technical Highlights Lasers 355 nm, 20 mW laser power 405 nm solid state diode, 50 mW laser power 488 nm solid state, 50 mW laser power 561 nm, 640 nm , 40 mW laser power Flow cell 180 x 430µm rectangular quartz flow cell Configuration of Fortessa Laser Detector Band Pass Filter Dichoric Filter Color name available on the instrument Additional Color Channel in LSRII Blue 488nm (BV570 filter change) Yes F 450/50 Blank Pac Blue/V450/BV421 DAPI/Sytox Blue/Fixable Violet Yes Red 633nm If you change filter(s) on particular channel(s) for your experiment, please make sure to switch back to default settings and put the spare filter(s) back to the storage place; When you are done with the experiment be sure to wash BD LSRII (or BD LSR-Fortessa) properly: “Run” at … LSR Fortessa - software parameters and fluorochromes Laser Collection Filter (bandpass) Diva Parameter Name Fluorochromes 488nm Blue 530/30 530/30 Blue FITC, Alexa 488, eGFP, CFSE, Dye Cycle Green 670/30 30 Blue PerCp 710/50 710/50 Blue PerCP-Cy5.5, PerCp-eFluor 710, PerCp provide easier access to bandpass filters and mirrors, simplifying changes to experimental setup. To improve experimental efficiency, the optional BD™ High Throughput Sampler (HTS) allows sample loading from 96- or 384-well microtiter plates for screening assays.

BD LSRFortessa X-20 Configuration Laser Detector Filter Mirror Dye 488nm (Blue) A 695/40 670LP PerCP, PerCP-Cy5.5 PerCP efluor 710 B 530/30 505LP FITC, GFP, CFSE, BB515 Alexa Fluor 488 C 488/10 - SSC 561nm (Yellow/Green) A 780/60 750LP PE-Cy7 B 670/30 635LP

Detector Name. Preceding BV510, BD Horizon V500, Pacific. Orange, Qdot525. BD Fortessa X20 is a state - of - the - art analyser equipped with 5 spatially spectrum is covered by optical filters, however if you need different filter setup for LSR Fortessa Overview Check saline filter for air bubbles and vent if necessary . Vent Start the BD FACSDiva software program by double-‐clicking. Each signal is directed through an array of mirrors and filters to a specific detector .

Leave the support arm to the side. 2) Choose “New Experiment” . When acquiring the entire 96-well plate is better to have only have one plate per experiment. … 2015-05-26 The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency.
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Be aware that the lasers take about 15 minutes to warm up and BD LSR Fortessa Standard Operation Protocol Refill Sheath Container 1. Make sure the flow cytometer is in STANDBY mode. 2.

5. Designed for limited space and boundless potential. The BD LSRFortessa™ X-20 cell analyzer delivers high-performance multicolor analysis with the most compact footprint in its class at just 30" x 29" (76.2 x 73.7 cm) and a height of 30" (76.2 cm).
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• Log in to BD FACSDiva software MACHINE SETUP: • Turn on the machine if you are the first user of the day. There is no fluidics startup for the Fortessa. • Give the lasers 20-30 minutes of warm-up time. • Check tank levels for FACS buffer and waste o To fill FACS buffer unscrew probe and place in holster. Silence alarm by clicking

Venus BD Horizon V450. BV421.

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The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be affordable and expandable, the BD LSRFortessa

If you are getting events continue washing with FACSClean BD, BD Logo and all other trademarks are the property of Becton, Dickinson and Company. ©2006 BD 23-8641-00 BD Biosciences Technical Specifications BD™ LSR II The Most Flexible Flow Cytometer Available. Performance Fluorescence Sensitivity Measurements performed using SPHERO™ Rainbow Calibration Particles RCP-30-5A. Fortessa A. 355nM UV. Collection filter Mirror Fluorochromes; 450/50- Alexa Fluor 405, BD Horizon V450, BV 421, Calcein Violet, Calcein Violet 450AM, Core Facility, BBB BD LSR Fortessa, filter configurations and fluorochromes Marianne Enger Side 1 12.06.2013 LASER PMT LP FILTER BP FILTER FLUOROCHROMES (examples) A 685 695/40 PerCP-Cy5.5 488 (blue) D B 635 670/30 PerCP C 505 530/30 FITC, eGFP, eYFP, DiO, CFSE, YoPro1, Alexa488 - 488/10 SSC FSC 1 inch filter cassette v10.x3d Rating Select rating Give BD Aria/LSRII/Fortessa optical filter holders 1/5 Give BD Aria/LSRII/Fortessa optical filter holders 2/5 Give BD Aria/LSRII/Fortessa optical filter holders 3/5 Give BD Aria/LSRII/Fortessa optical filter holders 4/5 BD FACSCelesta ™ multicolor cell analyzer . The BD FACSCelesta multicolor cell analyzer helps rapidly and accurately analyze cells in a compact, affordable package. With up to four different laser configurations and 14 parameters, the cytometer is fully optimized to work with BD Horizon Brilliant ™ dyes, so you can focus on what matters most—your research. 4) Start BD FACSDiva software.